Edexcel IGCSE Biology · Spec 5.12
DNA Ligase and Recombinant Plasmids
Covers distinguish restriction enzymes from DNA ligase, Explain how a recombinant plasmid is made and Describe how transformed bacteria are selected.
Biology revision video
DNA Ligase and Recombinant Plasmids
Prefer to watch on YouTube? Open this video on YouTube.
Spec 5.12
What you need to know
- Distinguish restriction enzymes from DNA ligase
- Explain how a recombinant plasmid is made
- Describe how transformed bacteria are selected
Active recall
Quick check
Answer each question before opening the answer.
What is the role of a restriction enzyme?
It cuts DNA at a specific recognition sequence
What is the role of DNA ligase?
It joins DNA fragments by bonding the sugar-phosphate backbone
Why is a marker gene useful?
It identifies or selects cells that received a plasmid
Biology video companion
Keep your notes and exam practice together
The free Biology workbook follows every video in specification order.