Edexcel IGCSE Biology · Spec 2.14B
Investigating Enzymes and pH
Required practical: investigating how pH affects the enzyme amylase, using buffers and iodine.
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Investigating Enzymes and pH
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Spec 2.14B
What you need to know
- Investigate how pH affects enzyme activity
- Follow the amylase and starch method
- Interpret the results
Active recall
Quick check
Answer each question before opening the answer.
How does pH affect enzyme activity?
Each enzyme has an optimum pH; moving away from it slows the reaction, and extreme pH denatures the enzyme.
How do you keep the pH of each reaction constant?
Use buffer solutions of the required pH.
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