Edexcel IGCSE Biology · Spec 2.12
Investigating Enzymes Practical
Required practical: investigating how temperature affects the enzyme amylase, using starch and iodine.
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Investigating Enzymes Practical
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Spec 2.12
What you need to know
- Investigate how temperature affects an enzyme
- Describe the method using amylase and starch
- Explain the results
Active recall
Quick check
Answer each question before opening the answer.
How can you tell when amylase has fully broken down starch?
Test samples with iodine at intervals; when it no longer turns blue-black, the starch has been digested.
Name two variables you must control in this experiment.
Any two of: pH, temperature, enzyme concentration, substrate concentration, volumes used.
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